Aminated Surfaces

Surfaces with primary or secondary amino groups covalently bound are dedicated to promote the covalent immobilisation of compounds containing reactive moieties such as amino, carboxyl or thiol groups via well-known homoheterobifunctional linkers, e.g. N-Hydroxysuccinimide (NHS) or Succinimidyl 4-(N-maleidomethyl) cyclohexane-1- carboxylate (SMCC).

This kind of immobilisation can overcome some of the limitations connected with physical adsorption of the molecules to the surfaces:

  • immobilisation of molecules which are bound weakly or not at all by physical adsorption, namely small peptides (M.W. 1000-5000) drugs, toxins or hormones.
  • oriented immobilisation of molecules in order to secure the integrity and accessibility of their specific sites avoiding the risk of inhibition of these sites by casual physical adsorption for such molecules as Fab-SH-antibody fragments, streptavidin, polysaccharides or nucleic acids (single strand or double strand)
  • increased storage stability compared with that of physical adsorption because of the reduced risk of spontaneous desorption.

    • Plates are tested and certified for:
    uniformity
    binding specificity
    amino groups activity