Jacalin Coated Surfaces

The Biomat product is a 96 well coated microplate with Jacalin and a surfactant to block non-specific binding sites and to maintain stable activity.

Jacalin, belonging to the lectins family, is a tetrameric ( MW 40.000 ) carbohydrate-binding protein isolated from the seeds of jack fruit Artrocarpus integrifolia agglutinin. It is well known that lectins have been used extensively for the isolation of glycol-conjugates and glycoproteins with specific carbohydrate structures.

Jacalin shows specific affinity for molecules containing non reducing α-D-galactosyl groups, usually present in the biochemical structure of IgA1 and cell membranes.

Example of applications:

Lots are tested and certified for

Product specifications

Components

Individually pouched 96-well microplates, configured in 12 removable 8-well strips or solid format

Coating

Jacalin ( mol. Weight 40 kDa ) , from the seeds of jack fruit Artrocarpus integrifolia, is coated using 100 µl/well. The strips are post-coated ( blocked ) for low non specific binding and long-term stability.

Binding capacity

Microplate was saturated with biotinylated human IgA at a concentration of 6 µg/ml ( 600 ng/well ) in an ELISA format using Streptavidin-HRP as detector and TMB as substrate ( see Figure 1 for data and experiment details ).

The Biomat Jacalin microplate shows a nominal binding capacity of ~ 4 pmol IgA/well.

Sensitivity

Biotinylated human IgA was detected at a concentration significantly above background in an ELISA format using streptavidin-HRP as detector and TMB as substrate ( see Figure 2 for data and experiment details ).

The Biomat Jacalin microplate shows a sensitivity of 0.0161 µg/ml ( 1.61 ng/well ) of human IgA.

Uniformity

Microplates show a CV% less than 5 when used as a catcher of biotinylated human IgA in an ELISA format using streptavidin-HRP as detector and TMB as substrate.

Storage and Stability

The microplates, if unopened, are stable refrigerated until the expiration date printed on the label. If opened, store in closed pouch with dessicant and use within the expiration date.